CRISPR GUI
Sign in with your Bioagens account to analyze CRISPR editing outcomes.
/ CRISPR Editing Analysis
CRISPR GUI
Upload amplicon sequencing reads (FASTQ), quantify NHEJ/HDR editing outcomes with CRISPResso2, and search genome-wide off-target sites with Cas-OFFinder.
Drag a .fastq/.fq(.gz) file here, or click to browse
If provided, CRISPResso classifies reads separately against this HDR-edited reference.
SpCas9 NGG PAM assumed. A full-genome search can take a couple of minutes.
Results will appear here.
Running CRISPResso2...
Editing frequency
| Amplicon | Unmodified % | Modified % | Reads aligned | Ins / Del / Sub |
|---|
Indel size distribution
bp relative to cut site (0 = no indel)
Off-target sites
| Chrom | Position | Sequence | Strand | Mismatches |
|---|